TOR-dependent reduction in the expression level of Rrn3p lowers the activity of the yeast RNA Pol I machinery, but does not account for the strong inhibition of rRNA production

Nucleic Acids Res. 2010 Sep;38(16):5315-26. doi: 10.1093/nar/gkq264. Epub 2010 Apr 25.

Abstract

Ribosome biogenesis is tightly linked to cellular growth. A crucial step in the regulation of ribosomal RNA (rRNA) gene transcription is the formation of the complex between RNA polymerase I (Pol I) and the Pol I-dependent transcription factor Rrn3p. We found that TOR inactivation leads to proteasome-dependent degradation of Rrn3p and a strong reduction in initiation competent Pol I-Rrn3p complexes affecting yeast rRNA gene transcription. Using a mutant expressing non-degradable Rrn3p or a strain in which defined endogenous Rrn3p levels can be adjusted by the Tet-off system, we can demonstrate that Rrn3p levels influence the number of Pol I-Rrn3p complexes and consequently rRNA gene transcription. However, our analysis reveals that the dramatic reduction of rRNA synthesis in the immediate cellular response to impaired TOR signalling cannot be explained by the simple down-regulation of Rrn3p and Pol I-Rrn3p levels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Genes, rRNA*
  • Pol1 Transcription Initiation Complex Proteins / analysis
  • Pol1 Transcription Initiation Complex Proteins / genetics
  • Pol1 Transcription Initiation Complex Proteins / metabolism*
  • Proteasome Endopeptidase Complex / metabolism
  • Protein Serine-Threonine Kinases / antagonists & inhibitors
  • Protein Serine-Threonine Kinases / metabolism*
  • RNA Polymerase I / metabolism*
  • RNA, Ribosomal / biosynthesis*
  • RNA, Ribosomal / genetics
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae Proteins / analysis
  • Saccharomyces cerevisiae Proteins / antagonists & inhibitors
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Sequence Deletion
  • Sirolimus / pharmacology
  • Transcription, Genetic
  • Ubiquitination

Substances

  • Pol1 Transcription Initiation Complex Proteins
  • RNA, Ribosomal
  • RRN3 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Protein Serine-Threonine Kinases
  • target of rapamycin protein, S cerevisiae
  • RNA Polymerase I
  • Proteasome Endopeptidase Complex
  • Sirolimus