Therapeutic vaccination against a murine lymphoma by intratumoral injection of a cationic anticancer peptide

Cancer Immunol Immunother. 2010 Aug;59(8):1285-94. doi: 10.1007/s00262-010-0857-6. Epub 2010 Apr 27.


Cationic antimicrobial peptides (CAPs) exhibit promising anticancer activities. In the present study, we have examined the in vivo antitumoral effects of a 9-mer peptide, LTX-302, which is derived from the CAP bovine lactoferricin (LfcinB). A20 B cell lymphomas of BALB/c origin were established by subcutaneous inoculation in syngeneic mice. Intratumoral LTX-302 injection resulted in tumor necrosis and infiltration of inflammatory cells followed by complete regression of the tumors in the majority of the animals. This effect was T cell dependent, since the intervention was inefficient in nude mice. Successfully treated mice were protected against rechallenge with A20 cells, but not against Meth A sarcoma cells. Tumor resistance could be adoptively transferred with spleen cells from LTX-302-treated mice. Resistance was abrogated by depletion of T lymphocytes, or either the CD4(+) or CD8(+) T cell subsets. Taken together, these data suggest that LTX-302 treatment induced long-term, specific cellular immunity against the A20 lymphoma and that both CD4(+) and CD8(+) T cells were required. Thus, intratumoral administration of lytic peptide might, in addition to providing local tumor control, confer a novel strategy for therapeutic vaccination against cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Animals
  • Antimicrobial Cationic Peptides / administration & dosage*
  • Antimicrobial Cationic Peptides / chemistry
  • Cancer Vaccines*
  • Cattle
  • Cell Line, Tumor
  • Female
  • HMGB1 Protein / metabolism
  • Lactoferrin / administration & dosage*
  • Lactoferrin / chemistry
  • Lymphocyte Activation / drug effects
  • Lymphocyte Depletion
  • Lymphoma, B-Cell / immunology*
  • Lymphoma, B-Cell / pathology
  • Lymphoma, B-Cell / therapy
  • Mice
  • Mice, Inbred BALB C
  • Microscopy, Electron, Scanning
  • Neoplasm Transplantation
  • Peptide Fragments / administration & dosage*
  • Peptide Fragments / chemistry
  • Remission Induction
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism
  • T-Lymphocytes / pathology
  • Tumor Burden / drug effects


  • Antimicrobial Cationic Peptides
  • Cancer Vaccines
  • HMGB1 Protein
  • Peptide Fragments
  • lactoferricin B
  • Lactoferrin