Agonistic behavior and electrical stimulation of the antennae induces Fos-like protein expression in the male cricket brain

Arch Insect Biochem Physiol. 2010 May;74(1):38-51. doi: 10.1002/arch.20360.

Abstract

Immediate early genes (IEG) such as c-Fos and Fos-related antigens (FRA) have been used as markers of neuronal activation. In this study, we determined whether the expression of c-Fos/FRAs is increased in the brains of adult male Acheta domesticus crickets following agonistic interactions. We looked for c-Fos/FRA proteins in the brain of un-fought, control male crickets and of dominant and subordinate male crickets sacrificed at different time periods following an agonistic interaction. Using immunoblot analysis, we found four different c-Fos/FRA-like proteins in the adult cricket brain. Continuous agonistic interaction increased c-Fos/FRA protein expression in the brains of subordinate males compared to control and dominant males. In addition, direct electrical stimulation of the male cricket antennae increased c-Fos/FRA-like protein in the brain. We identified the specific brain regions that exhibit c-Fos/FRA-like immunoreactivity in crickets. We detected c-Fos/FRA-like cellular immunoreactivity in different functional regions of the adult brain including the pars intercerebralis, protocerebrum, deutocerebrum, and the cortex of the mushroom bodies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agonistic Behavior / physiology*
  • Animals
  • Behavior, Animal / physiology*
  • Brain / metabolism
  • Electric Stimulation*
  • Gene Expression Profiling
  • Gene Expression Regulation / physiology*
  • Gryllidae / metabolism
  • Gryllidae / physiology*
  • Immunohistochemistry
  • Male
  • Netrin Receptors
  • Proto-Oncogene Proteins c-fos / metabolism*
  • Receptors, Cell Surface / metabolism

Substances

  • Netrin Receptors
  • Proto-Oncogene Proteins c-fos
  • Receptors, Cell Surface