The regulation of jejunal induction of the maltase-glucoamylase gene by a high-starch/low-fat diet in mice

Mol Nutr Food Res. 2010 Oct;54(10):1445-51. doi: 10.1002/mnfr.200900467.

Abstract

Maltase and glucoamylase are derived from the same mRNA and are responsible for digestion of starch in the small intestine. Their jejunal activities in rodents are induced by a high-starch/low-fat (HS)-diet. However, it is unknown whether jejunal expression of the maltase-glucoamylase (Mgam) gene is enhanced by the HS-diet. In this study, we found that jejunal Mgam mRNA was increased by a HS-diet in mice. We showed that the HS-diet increased acetylation of histones, bindings of a coactivator, Creb binding protein (CREBBP), and the transcriptional factors caudal type homeobox 2 (CDX2) and HNF1 homeobox (HNF1) in the promoter/enhancer and transcriptional regions of Mgam gene. This suggests that the increase in the jejunal activity of maltase and glucoamylase caused by a HS-diet in mice is regulated at the mRNA level through histone acetylation and binding of CREBBP, CDX2 and HNF1 in the promoter/enhancer and transcriptional regions of Mgam gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Animals
  • CDX2 Transcription Factor
  • CREB-Binding Protein / metabolism
  • DNA-Binding Proteins / metabolism
  • Diet* / adverse effects
  • Diet, Fat-Restricted
  • Enzyme Induction*
  • Hepatocyte Nuclear Factor 1 / metabolism
  • Histones / metabolism
  • Homeodomain Proteins / metabolism
  • Jejunum / enzymology*
  • Jejunum / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Open Reading Frames
  • Promoter Regions, Genetic
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Starch / administration & dosage*
  • Starch / adverse effects
  • Transcription Factors / metabolism
  • alpha-Glucosidases / biosynthesis*
  • alpha-Glucosidases / genetics

Substances

  • CDX2 Transcription Factor
  • Cdx2 protein, mouse
  • DNA-Binding Proteins
  • Histones
  • Homeodomain Proteins
  • RNA, Messenger
  • Transcription Factors
  • Hepatocyte Nuclear Factor 1
  • Starch
  • CREB-Binding Protein
  • Crebbp protein, mouse
  • alpha-Glucosidases