Whole-animal senescent cytotoxic T cell removal using antibodies linked to magnetic nanoparticles

Rejuvenation Res. Apr-Jun 2010;13(2-3):298-300. doi: 10.1089/rej.2009.0964.

Abstract

A major type of unwanted cells that accumulate in aging are anergic cytotoxic T cells. These cells often have virus-specific T cell receptors, as well as other surface markers that distinguish them from their youthful counterparts, and they are thought to play a major role in the decline of the immune system with age. Here we consider two surface markers thought to define these cells in mice, CD8 and Killer cell lectin-like receptor G1 (KLRG1), and a means we developed to remove these cells from the blood of aged C57BL/6 mice. Using antibodies with magnetic nanoparticles linked to their Fc domains, we first developed a method to use magnets to filter out the unwanted cells from the blood and later constructed a device that does this automatically. We demonstrated that this device could reduce the KLRG1-positive CD8 cell count in aged mouse blood by a factor of 7.3 relative to the total CD8 cell compartment, reaching a level typically seen only in very young animals.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / immunology*
  • Aging / pathology
  • Animals
  • Antilymphocyte Serum / chemistry
  • Antilymphocyte Serum / therapeutic use*
  • CD8-Positive T-Lymphocytes / pathology
  • Cell Separation / methods
  • Cytapheresis / methods
  • Magnetics
  • Mice
  • Mice, Inbred C57BL
  • Nanoparticles / chemistry
  • Nanoparticles / therapeutic use*
  • Receptors, Immunologic / genetics
  • Receptors, Immunologic / metabolism
  • T-Lymphocytes, Cytotoxic / pathology*

Substances

  • Antilymphocyte Serum
  • Klrg1 protein, mouse
  • Receptors, Immunologic