The biomass objective function

Curr Opin Microbiol. 2010 Jun;13(3):344-9. doi: 10.1016/j.mib.2010.03.003. Epub 2010 Apr 27.


Flux balance analysis (FBA) is a mathematical approach for analyzing the flow of metabolites through a metabolic network. To computationally predict cell growth using FBA, one has to determine the biomass objective function that describes the rate at which all of the biomass precursors are made in the correct proportions. Here we review fundamental issues associated with its formulation and use to compute optimal growth states.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Biomass*
  • Cell Growth Processes*
  • Energy Metabolism
  • Escherichia coli / growth & development*
  • Escherichia coli / metabolism*
  • Eukaryotic Cells
  • Hybridomas / metabolism*
  • Metabolic Networks and Pathways
  • Metabolome
  • Models, Theoretical
  • Saccharomyces cerevisiae / growth & development*
  • Saccharomyces cerevisiae / metabolism*
  • Systems Biology