Abstract
Microfluorimetry of fura-2 was used to monitor [Ca2+]i in single cells stimulated with the G-protein activating agent mastoparan. Mastoparan induced the generation of [Ca2+]i oscillations, which in contrast to oscillations induced by low concentrations of CCK were acutely dependent on the presence of extracellular Ca2+. Oscillations were inhibited by phorbol ester. Sodium fluoride, a known activator of G-proteins, gave similar results. Both mastoparan and CCK induced turnover of inositol phosphates, at concentrations higher than necessary to induce oscillations.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alkaloids / pharmacology
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Animals
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Calcium / metabolism*
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Cholecystokinin / pharmacology
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Cytosol / drug effects
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Cytosol / metabolism
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In Vitro Techniques
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Inositol Phosphates / metabolism
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Intercellular Signaling Peptides and Proteins
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Kinetics
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Male
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Microscopy, Fluorescence
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Pancreas / cytology
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Pancreas / drug effects
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Pancreas / metabolism*
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Peptides
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Protein Kinase C / antagonists & inhibitors
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Protein Kinase C / metabolism
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Rats
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Sodium Fluoride / pharmacology
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Staurosporine
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Tetradecanoylphorbol Acetate / pharmacology
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Time Factors
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Wasp Venoms / pharmacology*
Substances
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Alkaloids
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Inositol Phosphates
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Intercellular Signaling Peptides and Proteins
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Peptides
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Wasp Venoms
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mastoparan
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Sodium Fluoride
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Cholecystokinin
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Protein Kinase C
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Staurosporine
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Tetradecanoylphorbol Acetate
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Calcium