A gene structure of testosterone 6 beta-hydroxylase (P450IIIA)

Biochem Biophys Res Commun. 1991 May 31;177(1):68-73. doi: 10.1016/0006-291x(91)91949-d.

Abstract

Genomic clones of a rat testosterone 6 beta-hydroxylase have been isolated and characterized as the first gene (P450/6 beta A) among P450IIIA subfamily. This gene spans about 25Kb and consists of 13 exons, which is the largest number of exons among cytochrome P-450 genes reported previously. The nucleotide sequence of the exon region showed high similarity to those of P450PCN2 and P450PCN1 cDNA (Gonzalez, F.J. et al. (1987) Mol. Cell. Biol. 2969-2974), but several replacements and deletions of nucleotide were found between the P450/6 beta A gene and both cDNAs, indicating the existence of multiple P450IIIA genes in rats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cytochrome P-450 Enzyme System / genetics*
  • Exons
  • Genes*
  • Genomic Library
  • Introns
  • Molecular Sequence Data
  • Multigene Family
  • Oligonucleotide Probes
  • Rats
  • Rats, Inbred Strains
  • Restriction Mapping
  • Steroid Hydroxylases / genetics*
  • TATA Box
  • Transcription, Genetic

Substances

  • Oligonucleotide Probes
  • Cytochrome P-450 Enzyme System
  • Steroid Hydroxylases
  • steroid hormone 6-beta-hydroxylase

Associated data

  • GENBANK/M62480
  • GENBANK/M62481
  • GENBANK/M62482
  • GENBANK/M74443
  • GENBANK/M75163
  • GENBANK/M75164
  • GENBANK/S38487
  • GENBANK/S38493
  • GENBANK/S38494
  • GENBANK/X53944