Abstract
Genomic clones of a rat testosterone 6 beta-hydroxylase have been isolated and characterized as the first gene (P450/6 beta A) among P450IIIA subfamily. This gene spans about 25Kb and consists of 13 exons, which is the largest number of exons among cytochrome P-450 genes reported previously. The nucleotide sequence of the exon region showed high similarity to those of P450PCN2 and P450PCN1 cDNA (Gonzalez, F.J. et al. (1987) Mol. Cell. Biol. 2969-2974), but several replacements and deletions of nucleotide were found between the P450/6 beta A gene and both cDNAs, indicating the existence of multiple P450IIIA genes in rats.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Cytochrome P-450 Enzyme System / genetics*
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Exons
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Genes*
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Genomic Library
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Introns
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Molecular Sequence Data
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Multigene Family
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Oligonucleotide Probes
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Rats
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Rats, Inbred Strains
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Restriction Mapping
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Steroid Hydroxylases / genetics*
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TATA Box
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Transcription, Genetic
Substances
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Oligonucleotide Probes
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Cytochrome P-450 Enzyme System
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Steroid Hydroxylases
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steroid hormone 6-beta-hydroxylase
Associated data
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GENBANK/M62480
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GENBANK/M62481
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GENBANK/M62482
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GENBANK/M74443
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GENBANK/M75163
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GENBANK/M75164
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GENBANK/S38487
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GENBANK/S38493
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GENBANK/S38494
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GENBANK/X53944