Degradation of nicotine in tobacco waste extract by newly isolated Pseudomonas sp. ZUTSKD

Bioresour Technol. 2010 Sep;101(18):6935-41. doi: 10.1016/j.biortech.2010.03.142.

Abstract

The newly isolated Pseudomonas sp. ZUTSKD was evaluated for its ability to degrade nicotine in tobacco waste extract (TWE). The strain degraded nicotine completely when the concentration of reducing sugar in TWE was lower than 8 gL(-1). Yeast extract and phosphate additions improved nicotine degradation in 5% TWE. At 30 degrees C and pH 7.0, with additional 15 gL(-1) Na(2)HPO(4).6H(2)O and 6 gL(-1) KH(2)PO(4) in 5% TWE, Pseudomonas sp. ZUTSKD could degrade 97% of nicotine (1.6 gL(-1)) in 12h. The data showed that strain ZUTSKD could be useful to control the nicotine content in TWE.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biodegradation, Environmental
  • Industrial Waste / prevention & control*
  • Nicotiana / microbiology*
  • Nicotine / metabolism*
  • Pseudomonas / classification
  • Pseudomonas / isolation & purification*
  • Pseudomonas / metabolism*
  • Species Specificity

Substances

  • Industrial Waste
  • Nicotine