Structural characterization of the predominant family of histidine kinase sensor domains

J Mol Biol. 2010 Jul 16;400(3):335-53. doi: 10.1016/j.jmb.2010.04.049. Epub 2010 May 8.

Abstract

Histidine kinase (HK) receptors are used ubiquitously by bacteria to monitor environmental changes, and they are also prevalent in plants, fungi, and other protists. Typical HK receptors have an extracellular sensor portion that detects a signal, usually a chemical ligand, and an intracellular transmitter portion that includes both the kinase domain itself and the site for histidine phosphorylation. While kinase domains are highly conserved, sensor domains are diverse. HK receptors function as dimers, but the molecular mechanism for signal transduction across cell membranes remains obscure. In this study, eight crystal structures were determined from five sensor domains representative of the most populated family, family HK1, found in a bioinformatic analysis of predicted sensor domains from transmembrane HKs. Each structure contains an inserted repeat of PhoQ/DcuS/CitA (PDC) domains, and similarity between sequence and structure is correlated across these and other double-PDC sensor proteins. Three of the five sensors crystallize as dimers that appear to be physiologically relevant, and comparisons between ligated structures and apo-state structures provide insights into signal transmission. Some HK1 family proteins prove to be sensors for chemotaxis proteins or diguanylate cyclase receptors, implying a combinatorial molecular evolution.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Crystallography, X-Ray
  • Dimerization
  • Histidine Kinase
  • Methanosarcina / enzymology*
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Conformation
  • Protein Kinases / chemistry*
  • Protein Structure, Quaternary
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Rhodopseudomonas / enzymology*
  • Sequence Alignment
  • Shewanella / enzymology*
  • Vibrio parahaemolyticus / enzymology*

Substances

  • Protein Kinases
  • Histidine Kinase