Methylovulum Miyakonense Gen. Nov., Sp. Nov., a Type I Methanotroph Isolated From Forest Soil

Int J Syst Evol Microbiol. 2011 Apr;61(Pt 4):810-815. doi: 10.1099/ijs.0.019604-0. Epub 2010 Apr 30.

Abstract

A novel methanotroph, designated strain HT12(T), was isolated from forest soil in Japan. Cells of strain HT12(T) were Gram-reaction-negative, aerobic, non-motile, coccoid and formed pale-brown colonies. The strain grew only with methane and methanol as sole carbon and energy sources. Cells grew at 5-34 °C (optimum 24-32 °C). The strain possessed both particulate and soluble methane monooxygenases and assimilated formaldehyde using the ribulose monophosphate pathway. The major cellular fatty acids were C(16 : 0) (46.9 %) and C(14 : 0) (34.2 %), whereas unsaturated C(16) fatty acids, typical of type I methanotrophs, were absent. Comparative 16S rRNA gene sequence analysis showed that the most closely related strains were Methylosoma difficile LC 2(T) (93.1 % sequence similarity) and Methylobacter tundripaludum SV96(T) (92.6 % similarity). Phylogenetic analysis based on the pmoA gene indicated that strain HT12(T) formed a distinct lineage within the type I methanotrophs and analysis of the deduced pmoA amino acid sequence of strain HT12(T) showed that it had a 7 % divergence from that of its most closely related species. The DNA G+C content was 49.3 mol%. Based on this evidence, strain HT12(T) represents a novel species and genus of the family Methylococcaceae, for which the name Methylovulum miyakonense gen. nov., sp. nov. is proposed. The type strain of the type species is HT12(T) ( = NBRC 106162(T) = DSM 23269(T) = ATCC BAA-2070(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aerobiosis
  • Base Composition
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Formaldehyde / metabolism
  • Japan
  • Methane / metabolism
  • Methanol / metabolism
  • Methylococcaceae / classification*
  • Methylococcaceae / genetics
  • Methylococcaceae / isolation & purification*
  • Methylococcaceae / physiology
  • Molecular Sequence Data
  • Oxygenases / metabolism
  • Phylogeny
  • Pigments, Biological / metabolism
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Temperature
  • Trees

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Pigments, Biological
  • RNA, Ribosomal, 16S
  • Formaldehyde
  • Oxygenases
  • methane monooxygenase
  • Methane
  • Methanol

Associated data

  • GENBANK/AB501285
  • GENBANK/AB501286
  • GENBANK/AB501287
  • GENBANK/AB501290
  • GENBANK/AB524080