Abstract
Methods for the extraction of PCR-quality DNA from environmental soil samples by using pairs of commercially available kits were evaluated. Coxiella burnetii DNA was detected in spiked soil samples at <1,000 genome equivalents per gram of soil and in 12 (16.4%) of 73 environmental soil samples.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Coxiella burnetii / genetics
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Coxiella burnetii / isolation & purification
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DNA, Bacterial / analysis
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DNA, Bacterial / isolation & purification*
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Polymerase Chain Reaction / methods*
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Reagent Kits, Diagnostic
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Soil / analysis*
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Soil Microbiology*
Substances
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DNA, Bacterial
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Reagent Kits, Diagnostic
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Soil