Each Conserved Active Site Tyr in the Three Subunits of Human Isocitrate Dehydrogenase Has a Different Function

J Biol Chem. 2010 Jul 2;285(27):20520-5. doi: 10.1074/jbc.M110.115386. Epub 2010 Apr 30.

Abstract

The human NAD-dependent isocitrate dehydrogenase (IDH) is a heterotetrameric mitochondrial enzyme with 2alpha:1beta:1gamma subunit ratio. The three subunits share 40-52% identity in amino acid sequence and each includes a tyrosine in a comparable position: alphaY126, betaY137, and gammaY135. To study the role of the corresponding tyrosines of each of the subunits of human NAD-IDH, the tyrosines were mutated (one subunit at a time) to Ser, Phe, or Glu. Enzymes were expressed with one mutant and two wild-type subunits. The results of characterization of the mutant enzymes suggest that betaY137 is involved in NAD binding and allosteric activation by ADP. The alphaY126 is required for catalytic activity and likely acts as a general acid in the reaction. The gammaY135 is also required for catalytic activity and may be involved in proper folding of the enzyme. The corresponding tyrosines in the three dissimilar subunits of NAD-IDH thus have distinctive functions.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Catalytic Domain
  • Circular Dichroism
  • Codon / genetics
  • Conserved Sequence
  • DNA Primers
  • Gene Expression Regulation, Enzymologic
  • Humans
  • Isocitrate Dehydrogenase / chemistry*
  • Isocitrate Dehydrogenase / genetics
  • Isocitrate Dehydrogenase / metabolism*
  • Kinetics
  • Mitochondria / enzymology
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • NAD / metabolism
  • Protein Subunits / chemistry
  • Protein Subunits / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • Codon
  • DNA Primers
  • Protein Subunits
  • Recombinant Proteins
  • NAD
  • Isocitrate Dehydrogenase