RT-PCR and statistical analyses of adeABC expression in clinical isolates of Acinetobacter calcoaceticus-Acinetobacter baumannii complex

Microb Drug Resist. 2010 Jun;16(2):87-9. doi: 10.1089/mdr.2009.0131.

Abstract

The relationship between expression of adeABC and minimal inhibitory concentration (MIC) of tigecycline was investigated by RT-PCR and statistical analyses in a population of 106 clinical isolates (MIC range, 0.0313-16 microg/ml) of Acinetobacter calcoaceticus-Acinetobacter baumannii complex. There was a statistically significant linear relationship (p < 0.0001) between log-transformed expression values and log-transformed MIC values, indicating that overexpression of AdeABC efflux pump is a prevalent mechanism for decreased susceptibility to tigecycline in A. calcoaceticus-A. baumannii complex.

MeSH terms

  • Acinetobacter Infections / microbiology*
  • Acinetobacter baumannii / drug effects*
  • Acinetobacter baumannii / genetics
  • Acinetobacter baumannii / isolation & purification
  • Acinetobacter baumannii / metabolism
  • Acinetobacter calcoaceticus / drug effects*
  • Acinetobacter calcoaceticus / genetics
  • Acinetobacter calcoaceticus / isolation & purification
  • Acinetobacter calcoaceticus / metabolism
  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Drug Resistance, Bacterial
  • Humans
  • Membrane Transport Proteins / genetics
  • Membrane Transport Proteins / metabolism*
  • Microbial Sensitivity Tests
  • Minocycline / analogs & derivatives*
  • Minocycline / pharmacology
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Tigecycline

Substances

  • AdeA protein, Acinetobacter baumannii
  • AdeB protein, Acinetobacter baumannii
  • AdeC protein, Acinetobacter baumannii
  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Membrane Transport Proteins
  • Tigecycline
  • Minocycline