Protection of fibroblasts (NIH-3T3) against oxidative damage by cyanidin-3-rhamnoglucoside isolated from fig fruits (Ficus carica L.)

J Agric Food Chem. 2010 Jun 9;58(11):6660-5. doi: 10.1021/jf100122a.


Anthocyanins, plant secondary metabolites, have been recognized for their health-promoting properties when consumed by humans. In this study, the antioxidant properties of a major anthocyanin in fresh fig fruits, cyanidin-3-rhamnoglucoside (C3R), were evaluated by various assays in vitro and correlated with the protection afforded by C3R to cultured NIH-3T3 fibroblast cells. C3R inhibited lipid peroxidation from producing peroxy radicals (ROO(*)) and MDA in a dose-dependent manner, and a high calculated stoichiometric coefficient [n] for peroxy radicals was demonstrated. In addition to its scavenging of reactive oxygen species (ROS), C3R showed a strong chelating activity toward the Fe(2+) ion. Finally, pretreatment with C3R inhibited proapoptotic processes that were initiated by the oxidation of lysosome membranes in fibroblast cells. The high antioxidant potential, with several modes of action of purified C3R, may contribute to health benefits gained by the consumption of fresh fig fruits.

MeSH terms

  • Animals
  • Anthocyanins / isolation & purification
  • Anthocyanins / pharmacology*
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism*
  • Ficus / chemistry*
  • Mice
  • NIH 3T3 Cells
  • Oxidative Stress / drug effects*
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Protective Agents / isolation & purification
  • Protective Agents / pharmacology*


  • Anthocyanins
  • Plant Extracts
  • Protective Agents
  • cyanidin