Evolution: a guide to perturb protein function and networks

Curr Opin Struct Biol. 2010 Jun;20(3):351-9. doi: 10.1016/j.sbi.2010.04.002. Epub 2010 May 3.


Protein interactions give rise to networks that control cell fate in health and disease; selective means to probe these interactions are therefore of wide interest. We discuss here Evolutionary Tracing (ET), a comparative method to identify protein functional sites and to guide experiments that selectively block, recode, or mimic their amino acid determinants. These studies suggest, in principle, a scalable approach to perturb individual links in protein networks.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Amino Acid Sequence
  • Computational Biology
  • Evolution, Molecular*
  • GTP-Binding Proteins / genetics
  • GTP-Binding Proteins / metabolism
  • Humans
  • Protein Engineering
  • Proteins / chemistry
  • Proteins / genetics*
  • Proteins / metabolism*


  • Proteins
  • GTP-Binding Proteins