Role of BC Loop Residues in Structure, Function and Antigenicity of the West Nile Virus Envelope Protein Receptor-Binding Domain III

Virology. 2010 Jul 20;403(1):85-91. doi: 10.1016/j.virol.2010.03.038. Epub 2010 May 6.

Abstract

Site-directed mutagenesis of residues in the BC loop (residues 329-333) of the envelope (E) protein domain III in a West Nile virus (WNV) infectious clone and in plasmids encoding recombinant WNV and dengue type 2 virus domain III proteins demonstrated a critical role for residues in this loop in the function and antigenicity of the E protein. This included a strict requirement for the tyrosine at residue 329 of WNV for virus viability and E domain III folding. The absence of an equivalent residue in this region of yellow fever group viruses and most tick-borne flavivirus suggests there is an evolutionary divergence in the molecular mechanisms of domain III folding employed by different flaviviruses.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / isolation & purification
  • Antibodies, Neutralizing / immunology
  • Antibodies, Neutralizing / isolation & purification
  • Antibodies, Viral / immunology
  • Antibodies, Viral / isolation & purification
  • Antigens, Viral / genetics
  • Antigens, Viral / immunology
  • Antigens, Viral / physiology*
  • Chlorocebus aethiops
  • Female
  • Humans
  • Mice
  • Microbial Viability
  • Models, Molecular
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Neutralization Tests
  • Protein Folding
  • Protein Structure, Tertiary
  • Sequence Alignment
  • Vero Cells
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / immunology
  • Viral Envelope Proteins / physiology*
  • Virus Attachment*
  • West Nile virus / genetics
  • West Nile virus / immunology
  • West Nile virus / physiology*

Substances

  • Antibodies, Monoclonal
  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Antigens, Viral
  • Viral Envelope Proteins