Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography-mass spectrometry

Nat Protoc. 2010 Jun;5(6):986-92. doi: 10.1038/nprot.2010.37.


The ability to measure plant hormones quantitatively is important as plant hormones regulate plant growth, development and response to biotic and abiotic cues. In this protocol, we describe the quantitative analysis of major plant hormones from crude plant extracts. Plant hormones are determined using reverse-phase liquid chromatography-tandem mass spectrometry with multiple reaction monitoring. The method provides quantification of most major plant hormones in a single run from 50 mg of fresh plant tissue. Extraction and quantitative analysis of 40 samples takes 2-3 d.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / chemistry
  • Chromatography, High Pressure Liquid / methods*
  • Molecular Structure
  • Plant Extracts / chemistry*
  • Plant Growth Regulators / analysis*
  • Plant Growth Regulators / chemistry
  • Tandem Mass Spectrometry / methods*


  • Plant Extracts
  • Plant Growth Regulators