N-cadherin-associated proteins in chicken muscle

Differentiation. 1991 Feb;46(1):35-42. doi: 10.1111/j.1432-0436.1991.tb00863.x.

Abstract

The development and functional activity of the heart depends on the regulated interaction of cardiac cells. This is in part mediated by cell-cell adhesion molecules such as N-cadherin. N-cadherin belongs to a family of Ca+(+)-dependent, transmembrane, adhesion glycoproteins that promote cell-cell adhesion by molecular self-association extracellularly, and interact intracellularly with the cytoskeleton through highly conserved carboxy-terminal domains. In this paper we show that embryonic chicken cardiac myocytes grown in vitro display Ca+(+)-dependent adhesion and express N-cadherin. When immunoprecipitated from detergent extracts of embryonic chicken cardiac and skeletal muscle cultures, N-cadherin associates with proteins immunologically unrelated to itself. The associated proteins are similar in molecular weight to proteins that coimmunoprecipatate with E-cadherin from human epithelial cells. We postulate that the coimmunoprecipitating proteins are involved in linking the cadherins to the cytoskeleton.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cadherins / analysis*
  • Cell Aggregation
  • Cells, Cultured
  • Chick Embryo
  • Electrophoresis, Polyacrylamide Gel
  • Fluorescent Antibody Technique
  • Heart / embryology*
  • Immune Sera
  • Immunoblotting
  • Molecular Weight
  • Muscles / cytology
  • Muscles / embryology*
  • Myocardium / cytology

Substances

  • Cadherins
  • Immune Sera