FTIR study on the light sensitivity of the [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F: Ni-C to Ni-L photoconversion, kinetics of proton rebinding and H/D isotope effect

Phys Chem Chem Phys. 2009 Oct 21;11(39):8680-3. doi: 10.1039/b913635e. Epub 2009 Aug 18.

Abstract

The light-induced Ni-C to Ni-L transition results in the dissociation of a hydrogenic species, originating from the dihydrogen splitting at the active site. Back conversion in the dark to form Ni-C was investigated by studying the rebinding kinetics of this ligand in protonated (H(2)/H(2)O) and deuterated (D(2)/D(2)O) samples using time resolved FTIR spectroscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalytic Domain
  • Desulfovibrio vulgaris / enzymology*
  • Deuterium / chemistry*
  • Hydrogen / chemistry*
  • Hydrogenase / chemistry*
  • Hydrogenase / metabolism
  • Kinetics
  • Light*
  • Models, Molecular
  • Photochemistry
  • Protons*
  • Spectroscopy, Fourier Transform Infrared
  • Temperature
  • Water / chemistry

Substances

  • Protons
  • Water
  • Hydrogen
  • Deuterium
  • nickel-iron hydrogenase
  • Hydrogenase