[Studies on antinephritic effects of plant components in rats (1). Effects of saikosaponins original-type anti-GBM nephritis in rats and its mechanisms]

Nihon Yakurigaku Zasshi. 1991 Jan;97(1):13-21. doi: 10.1254/fpj.97.1_13.
[Article in Japanese]

Abstract

This study was designed to clarify the anti-nephritic effects of the saikosaponins that are contained in Bupleurum falcatum L. crude saikosaponin at 1.0 mg and 5.0 mg/kg, i.p. prevented urinary protein excretion and elevation of serum cholesterol content on the 10th day after the injection of anti-GBM serum. Moreover, crude saikosaponin at 1.0 mg and 5.0 mg/kg, i.p. significantly inhibited histopathological changes such as hypercellularity and adhesion. On the other hand, saikosaponin a (5.0 mg/kg, i.p.) and d (1.0 mg and 5.0 mg/kg, i.p.) also prevented urinary protein excretion, elevation of serum cholesterol content, and histopathological changes. In the second study, to clarify the anti-nephritic mechanisms of saikosaponins on this model, we investigated the effect of saikosaponins on platelet aggregation, release of corticosterone and reactive oxygen species scavengers activity. Crude saikosaponin and saikosaponin d significantly inhibited the increase in platelet aggregation, and saikosaponin d enhanced the serum and intra-adrenal corticosterone levels. Crude saikosaponin and saikosaponin a inhibited the decrease in activity of scavengers (SOD, catalase, glutathione peroxidase). These results indicate that saikosaponins were effective on this model, and anti-nephritic mechanisms of saikosaponins were party due to anti-platelet, corticosterone releasing and enhancing action on the activity of reactive oxygen species scavengers.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Cholesterol / blood
  • Corticosterone / blood
  • Free Radical Scavengers
  • Glomerulonephritis / drug therapy*
  • Glutathione Peroxidase / metabolism
  • Kidney / enzymology
  • Male
  • Oleanolic Acid* / analogs & derivatives*
  • Platelet Aggregation / drug effects
  • Platelet Aggregation Inhibitors / pharmacology
  • Platelet Aggregation Inhibitors / therapeutic use
  • Proteinuria
  • Rats
  • Rats, Inbred Strains
  • Sapogenins / pharmacology
  • Sapogenins / therapeutic use*
  • Saponins*
  • Superoxide Dismutase / metabolism

Substances

  • Free Radical Scavengers
  • Platelet Aggregation Inhibitors
  • Sapogenins
  • Saponins
  • Oleanolic Acid
  • Cholesterol
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • saikosaponin
  • Corticosterone