Mouse T-cell associated serine proteinase 1 degrades collagen type IV: a structural basis for the migration of lymphocytes through vascular basement membranes

Immunology. 1991 May;73(1):117-9.

Abstract

We show that CD8+ T-lymphocyte lines perferentially attach to collagen type IV and that mouse T-cell specific serine proteinase 1 (MTSP-1) preferentially degrades native basement membrane collagen type IV. In contrast, the interstitial collagen types I, II, III, V and VI appear not to be affected. The data reveal that MTSP-1 predominantly cleaves the alpha 2(IV) chain, which is found in the native triple helical structure of type IV collagen in a ratio of alpha 1(IV): alpha 2(IV) = 2:1 into small peptides. The cleavage of the alpha 2(IV) chain within the native collagen type IV molecules most likely results not only in a destabilization of single molecules but of the entire collagenous basement membrane scaffold at the site of MTSP-1 secretion.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Basement Membrane / metabolism
  • Cell Movement / immunology
  • Collagen / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Endothelium, Vascular / metabolism*
  • Mice
  • Serine Endopeptidases / physiology*
  • T-Lymphocytes / enzymology*
  • T-Lymphocytes / physiology

Substances

  • Collagen
  • Serine Endopeptidases