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. 2010 Sep;45(9):685-90.
doi: 10.1016/j.exger.2010.04.009. Epub 2010 May 6.

The Impact of Larval and Adult Dietary Restriction on Lifespan, Reproduction and Growth in the Mosquito Aedes Aegypti

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Free PMC article

The Impact of Larval and Adult Dietary Restriction on Lifespan, Reproduction and Growth in the Mosquito Aedes Aegypti

Teresa K Joy et al. Exp Gerontol. .
Free PMC article

Abstract

Dietary restriction extends lifespan in many organisms, but little is known about how it affects hematophagous arthropods. We demonstrated that diet restriction during either larval or adult stages extends Aedes aegypti lifespan. A. aegypti females fed either single or no blood meals survived 30-40% longer than those given weekly blood meals. However, mosquitoes given weekly blood meals produced far more eggs. To minimize reproduction's impact on lifespan, adult mosquitoes were fed artificial blood meals containing <10% of the protein in normal human blood, minimizing egg production. A. aegypti fed artificial blood meals containing 25mg/ml of BSA had significantly shorter lifespans than those fed either 10 or 5mg/ml. To assess the impact of larval dietary restriction on adult lifespan, we maintained larval A. aegypti on 2X, 1X (normal diet), 0.5X or 0.25X diets. Adult mosquitoes fed 0.5X and 0.25X larval diets survived significantly longer than those fed the 2X larval diet regardless of adult diet. In summary, dietary restriction during both larval and adult stages extends lifespan. This diet-mediated lifespan extension has important consequences for understanding how dietary restriction regulates lifespan and disease transmission.

Figures

Fig. 1
Fig. 1
The impact of blood feeding and dietary protein on adult A. aegypti lifespan. A. A representative survivorship curve comparing mosquitoes fed weekly blood meals (BM), a single blood meal, or no blood meals. All treatments were allowed access to 10% dextrose ad libitum. Dead individuals were counted daily. Letters following the figure legend indicate significant differences, with different letters indicating significant differences. B. Combined data showing the lifespan of adult female mosquitoes given access to an artificial blood meal containing 25, 10, 5 or 1 mg/ml of BSA every three days. All treatments were allowed ad libitum access to 10% dextrose. Survivorship was assessed daily. Treatments with the same letters were not different from each other; those with different letters have significant differences.
Fig. 2
Fig. 2
The effect of dietary restriction during the larval stages. A. Survivorship of mosquitoes fed 2X, 1X, 0.5X or 0.25X their normal diet as larvae and strictly sugar as adults. Letters following the figure legend indicate whether the differences between treatments were significant. Treatments with the same letters were not different from each other, while those with different letters had significant differences. B. Survivorship of mosquitoes fed 2X, 1X, 0.5X or 0.25X their normal diet as larvae and a single blood meal and 10% dextrose ad libitum as adults. Letters following the figure legend indicate significant differences, with different letters indicating significant differences. C. Survivorship of mosquitoes fed 2X, 1X, 0.5X or 0.25X their normal diet as larvae and multiple blood meals and 10% dextrose as adults. Letters following the figure legend indicate significant differences, with different letters indicating significant differences.
Fig. 3
Fig. 3
Egg production during successive reproductive cycles in A. aegypti and during the first reproductive cycles of aged mosquitoes. A. The number of eggs per cage laid by fully-fed females assayed for reproductive output on successive reproductive cycles. Different letters indicate a significant difference between treatments. B. Cohorts of adult mosquitoes aged 1, 2, 3, or 4 weeks old were given an identical blood meal and the number of eggs per cage was counted. Different letters indicate significant differences between treatments.

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