Optimization of medium for one-step fermentation of inulin extract from Jerusalem artichoke tubers using Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol

Bioresour Technol. 2010 Sep;101(18):7087-93. doi: 10.1016/j.biortech.2010.03.143. Epub 2010 May 7.

Abstract

The medium for one-step fermentation of raw inulin extract from Jerusalem artichoke tubers by Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol (R,R-2,3-BD) was developed. Inulin, K(2)HPO(4) and NH(4)Cl were found to be the key factors in the fermentation according to the results obtained from the Plackett-Burman experimental design. The optimal concentration range of the three factors was examined by the steepest ascent path, and their optimal concentration was further investigated according to the Box-Behnken design and determined to be 77.14 g/L, 3.09 g/L and 0.93 g/L, respectively. Under the optimal conditions, the concentration of the obtained R,R-2,3-BD was 36.92 g/L, at more than 98% optical purity. Compared with other investigated carbon resources, fermentation of the raw inulin extract afforded the highest yield of R,R-2,3-BD. This process featured one-step fermentation of inulin without further hydrolyzing, which greatly decreased the raw material cost and thus facilitated its practical application.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Butylene Glycols / isolation & purification
  • Butylene Glycols / metabolism*
  • Cell Culture Techniques / methods*
  • Computer Simulation
  • Helianthus / microbiology*
  • Insulin / metabolism*
  • Models, Biological*
  • Paenibacillus / classification
  • Paenibacillus / metabolism*
  • Plant Extracts / metabolism*
  • Plant Roots / metabolism*
  • Species Specificity

Substances

  • Butylene Glycols
  • Insulin
  • Plant Extracts