A call to standardize teratoma assays used to define human pluripotent cell lines

Franz-Josef Müller; Johanna Goldmann; Peter Löser; Jeanne F Loring

doi:10.1016/j.stem.2010.04.009

A call to standardize teratoma assays used to define human pluripotent cell lines

Cell Stem Cell. 2010 May 7;6(5):412-4. doi: 10.1016/j.stem.2010.04.009.

Authors

Franz-Josef Müller¹, Johanna Goldmann, Peter Löser, Jeanne F Loring

Affiliation

¹ Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA. fj.mueller@zip.kiel.de

PMID: 20452314
DOI: 10.1016/j.stem.2010.04.009

Abstract

The teratoma assay is the gold standard for documenting pluripotency of human stem cells. However, reports of new human ESC and iPSC lines vary widely in both methods and analysis of teratoma data. We call for consensus standards to be established to make this assay worthy of its "golden" status.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biological Assay / methods*
Biological Assay / standards*
Cell Line
Consensus
Humans
Meta-Analysis as Topic
Mice
Pluripotent Stem Cells / cytology*
Reference Standards
Teratoma / pathology*