Branched arabino-oligosaccharides isolated from sugar beet arabinan

Carbohydr Res. 2010 Jun 16;345(9):1180-9. doi: 10.1016/j.carres.2010.03.042. Epub 2010 Apr 4.


Sugar beet arabinan consists of an alpha-(1,5)-linked backbone of L-arabinosyl residues, which can be either single or double substituted with alpha-(1,2)- and/or alpha-(1,3)-linked L-arabinosyl residues. Neutral branched arabino-oligosaccharides were isolated from sugar beet arabinan by enzymatic degradation with mixtures of pure and well-defined arabinohydrolases from Chrysosporium lucknowense followed by fractionation based on size and analysis by MALDI-TOF MS and HPAEC. Using NMR analysis, two main series of branched arabino-oligosaccharides have been identified, both having an alpha-(1,5)-linked backbone of L-arabinosyl residues. One series carries single substituted alpha-(1,3)-linked L-arabinosyl residues at the backbone, whereas the other series consists of a double substituted alpha-(1,2,3,5)-linked arabinan structure within the molecule. The structures of eight such branched arabino-oligosaccharides were established.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabinose / chemistry*
  • Beta vulgaris / chemistry
  • Carbohydrate Sequence
  • Chrysosporium / enzymology
  • Dimerization
  • Hydrolases / metabolism
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Oligosaccharides / chemistry*
  • Oligosaccharides / isolation & purification*
  • Oligosaccharides / metabolism
  • Polysaccharides / chemistry*


  • Oligosaccharides
  • Polysaccharides
  • araban
  • Arabinose
  • Hydrolases