Abstract
We designed a new genetic tool to detect plasmid transfer under anaerobic and aerobic conditions. The system is based on the T7 RNA polymerase gene and a T7 promoter-driven oxygen-independent green fluorescent protein, evoglow, alone or in combination with red fluorescent protein DsRed. Constructs are available as plasmids and mini-mariner transposons.
Publication types
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Evaluation Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Aerobiosis
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Anaerobiosis
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Bacteriophage T7
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Conjugation, Genetic*
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DNA Transposable Elements
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DNA-Directed RNA Polymerases
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Escherichia coli / genetics*
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Escherichia coli / growth & development*
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Escherichia coli / virology
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Genetic Techniques
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Genetic Vectors*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Plasmids / genetics*
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Promoter Regions, Genetic
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Red Fluorescent Protein
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Viral Proteins
Substances
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DNA Transposable Elements
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Luminescent Proteins
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Viral Proteins
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Green Fluorescent Proteins
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bacteriophage T7 RNA polymerase
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DNA-Directed RNA Polymerases