Using poly(lactide-co-glycolide) electrospun scaffolds to deliver cultured epithelial cells to the cornea

Regen Med. 2010 May;5(3):395-401. doi: 10.2217/rme.10.16.


Aims: To assess the potential of electrospun poly(lactide-co-glycolide) membranes to provide a biodegradable cell carrier system for limbal epithelial cells.

Material & methods: 50:50 poly(lactide-co-glycolide) scaffolds were spun, sterilized and seeded with primary rabbit limbal epithelial cells. Cells were cultured on the scaffolds for 2 weeks and then examined by confocal microscopy, cryosectioning and scanning-electron microscopy. The tensile strength of scaffolds before and after annealing and sterilization was also studied.

Results: The limbal cells had formed a continuous multilayer of cells on either side of the scaffold. Scaffolds with cells showed signs of the onset of degradation within 2 weeks in culture media at 37 degrees C. Scaffolds that were annealed resulted in a more brittle and stiff mat.

Conclusions: We suggest this carrier membrane could be used as a replacement for the human amniotic membrane in the treatment of limbal stem cell deficiency, lowering the risk of disease transmission to the patient.

MeSH terms

  • Animals
  • Cell Culture Techniques
  • Cells, Cultured / cytology
  • Cornea / cytology*
  • Cryoultramicrotomy
  • Epithelial Cells / cytology*
  • Epithelial Cells / transplantation
  • Microscopy, Confocal / methods
  • Microscopy, Electron, Scanning / methods
  • Polyglactin 910 / chemistry*
  • Rabbits
  • Regenerative Medicine / methods*
  • Temperature
  • Time Factors
  • Tissue Engineering / methods
  • Tissue Scaffolds


  • Polyglactin 910