We present allele frequencies and statistical parameters of forensic interest for 10 autosomal STR loci and 12 Y-STR loci obtained from an Icelandic population sample. The testing of the STR loci in the AmpFlSTR SGM Plus kit in 151 unrelated individuals showed heterozygosity frequencies ranging from 0.775 (vWA) to 0.874 (D2S1338). A significant deviation from Hardy-Weinberg equilibrium was observed in vWA, but it was not statistically significant after application of Bonferroni correction. The exact test of differentiation analysis revealed one significant departure from differentiation out of 45 pairwise comparisons, but the departure was not significant after Bonferroni's correction. Seventy-five different haplotypes were observed in the 100 male samples analysed for the twelve Y-STRs included in the PowerPlex Y-system. No haplotype was observed more than four times. Pairwise comparisons for genetic distances based on the minimal haplotype diversity showed Iceland to be closer to Norway and Denmark than to Sweden, UK, Ireland and Greenland. As expected, the higher percentage of variation was observed within than among populations (90.40% versus 9.60%, respectively, for R(ST)).