A method for the isolation and purification of mouse peripheral blood monocytes

J Immunol Methods. 2010 Jul 31;359(1-2):1-10. doi: 10.1016/j.jim.2010.04.004. Epub 2010 May 8.


Studies in mice have advanced our understanding of the contribution of monocytes to inflammation and immunity. However, the lack of a straightforward and reliable method for the isolation of mouse blood monocytes remains a hurdle. Here we describe a fast and easy method for isolating monocytes from mouse blood based on immuno-magnetic labeling. By negative selection we were able to isolate enriched fractions (>50% purity) of unlabeled monocytes. A subsequent positive selection step resulted in purities of > or =90% viable and functional monocytes. This method is of general use for studying mouse monocyte biology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / immunology
  • Cells, Cultured
  • Female
  • Flow Cytometry
  • Immunomagnetic Separation / methods*
  • Leukocytes, Mononuclear / cytology*
  • Leukocytes, Mononuclear / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL


  • Antigens, CD