Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43

J Vet Sci. 2010 Jun;11(2):165-7. doi: 10.4142/jvs.2010.11.2.165.


Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Viral / immunology*
  • Blotting, Western
  • Coronavirus 229E, Human / immunology*
  • Coronavirus OC43, Human / immunology*
  • Cross Reactions
  • Humans
  • Nucleocapsid Proteins / genetics
  • Nucleocapsid Proteins / immunology*
  • Recombinant Proteins / immunology
  • Severe Acute Respiratory Syndrome / diagnosis
  • Severe Acute Respiratory Syndrome / immunology*
  • Severe acute respiratory syndrome-related coronavirus / genetics
  • Severe acute respiratory syndrome-related coronavirus / immunology*


  • Antibodies, Viral
  • Nucleocapsid Proteins
  • Recombinant Proteins