Feasibility of in vivo 15N MRS detection of hyperpolarized 15N labeled choline in rats

Phys Chem Chem Phys. 2010 Jun 14;12(22):5818-23. doi: 10.1039/c002309b. Epub 2010 May 12.


The increase of total choline in tumors has become an important biomarker in cancer diagnosis. Choline and choline metabolites can be measured in vivo and in vitro using multinuclear MRS. Recent in vivo(13)C MRS studies using labeled substrates enhanced via dynamic nuclear polarization demonstrated the tremendous potential of hyperpolarization for real-time metabolic studies. The present study demonstrates the feasibility of detecting hyperpolarized (15)N labeled choline in vivo in a rat head at 9.4 T. We furthermore report the in vitro (172 +/- 16 s) and in vivo (126 +/- 15 s) longitudinal relaxation times. We conclude that with appropriate infusion protocols it is feasible to detect hyperpolarized (15)N labeled choline in live animals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Choline / chemistry*
  • Isotope Labeling
  • Magnetic Resonance Imaging / methods*
  • Male
  • Nitrogen Isotopes / chemistry
  • Rats
  • Rats, Sprague-Dawley


  • Nitrogen Isotopes
  • Choline