Yaniella soli sp. nov., a new actinobacterium isolated from non-saline forest soil in China

Antonie Van Leeuwenhoek. 2010 Oct;98(3):395-401. doi: 10.1007/s10482-010-9453-1. Epub 2010 May 12.


A novel Gram-stain-positive, slightly halophilic, facultatively alkaliphilic, non-motile, non-sporulating, catalase-positive, oxidase-negative, aerobic bacterium, designated strain JSM 070026(T), was isolated from non-saline forest soil in China. Growth occurred with 0-20% (w/v) NaCl (optimum, 2-4%) and at pH 6.0-10.5 (optimum, pH 8.0) and 5-40 degrees C (optimum, 30 degrees C). Good growth also occurred in the presence of 0-28% (w/v) KCl (optimum, 2-5%) or 0-25% (w/v) MgCl(2)*6H(2)O (optimum, 1-4%). The peptidoglycan type was A4alpha (L: -Lys-Gly-L: -Glu). Cell-wall sugars contained mannose and xylose. The major cellular fatty acids were anteiso-C15:0 and iso-C15:0. Strain JSM 070026(T) contained menaquinone 8 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the major polar lipids. The DNA G + C content of strain JSM 070026(T) was 56.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JSM 070026(T) was a member of the suborder Micrococcineae and most closely related to Yaniella flava YIM 70178(T) (sequence similarity 99.4%) and Yaniella halotolerans YIM 70085(T) (97.9%). The three strains formed a distinct branch in the phylogenetic tree. The combination of phylogenetic analysis, DNA-DNA relatedness values, phenotypic characteristics and chemotaxonomic data supports the proposal that strain JSM 070026(T) represents a novel species of the genus Yaniella, for which the name Yaniella soli sp. nov. is proposed. The type strain is JSM 070026(T) (=DSM 22211(T) = KCTC 13527(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • China
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / analysis
  • DNA, Ribosomal / genetics
  • Genes, rRNA
  • Micrococcaceae / classification*
  • Micrococcaceae / genetics
  • Micrococcaceae / isolation & purification*
  • Micrococcaceae / physiology
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phylogeny
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Sodium Chloride
  • Soil Microbiology*
  • Trees / microbiology*


  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S
  • Sodium Chloride

Associated data

  • GENBANK/FJ527420