The effect of marathon on mRNA expression of anti-apoptotic and pro-apoptotic proteins and sirtuins family in male recreational long-distance runners

BMC Physiol. 2010 May 12;10:7. doi: 10.1186/1472-6793-10-7.

Abstract

Background: A large body of evidence shows that a single bout of strenuous exercise induces oxidative stress in circulating human lymphocytes leading to lipid peroxidation, DNA damage, mitochondrial perturbations, and protein oxidation.In our research, we investigated the effect of physical load on the extent of apoptosis in primary cells derived from blood samples of sixteen healthy amateur runners after marathon (a.m.).

Results: Blood samples were collected from ten healthy amateur runners peripheral blood mononuclear cells (PBMCs) were isolated from whole blood and bcl-2, bax, heat shock protein (HSP)70, Cu-Zn superoxide dismutase (SOD), Mn-SOD, inducible nitric oxide synthase (i-NOS), SIRT1, SIRT3 and SIRT4 (Sirtuins) RNA levels were determined by Northern Blot analysis. Strenuous physical load significantly increased HSP70, HSP32, Mn-SOD, Cu-Zn SOD, iNOS, GADD45, bcl-2, forkhead box O (FOXO3A) and SIRT1 expression after the marathon, while decreasing bax, SIRT3 and SIRT4 expression (P < 0.0001).

Conclusion: These data suggest that the physiological load imposed in amateur runners during marathon attenuates the extent of apoptosis and may interfere with sirtuin expression.

Publication types

  • Retracted Publication

MeSH terms

  • Apoptosis Regulatory Proteins / genetics*
  • Cell Cycle Proteins / genetics
  • Forkhead Box Protein O3
  • Forkhead Transcription Factors / genetics
  • HSP70 Heat-Shock Proteins / genetics
  • Heme Oxygenase-1 / genetics
  • Humans
  • Lipid Peroxidation / physiology
  • Lymphocytes / physiology
  • Male
  • Mitochondrial Proteins / genetics
  • Nitric Oxide Synthase Type II / genetics
  • Nuclear Proteins / genetics
  • Oxidative Stress / physiology
  • Physical Endurance / physiology*
  • Proto-Oncogene Proteins c-bcl-2 / genetics
  • RNA, Messenger / metabolism
  • Running / physiology*
  • Signal Transduction / genetics
  • Sirtuin 1 / genetics
  • Sirtuin 3 / genetics
  • Sirtuins / genetics*
  • Superoxide Dismutase / genetics
  • Superoxide Dismutase / metabolism
  • bcl-2-Associated X Protein / genetics

Substances

  • Apoptosis Regulatory Proteins
  • BAX protein, human
  • Cell Cycle Proteins
  • FOXO3 protein, human
  • Forkhead Box Protein O3
  • Forkhead Transcription Factors
  • GADD45A protein, human
  • HSP70 Heat-Shock Proteins
  • Mitochondrial Proteins
  • Nuclear Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • RNA, Messenger
  • bcl-2-Associated X Protein
  • NOS2 protein, human
  • Nitric Oxide Synthase Type II
  • HMOX1 protein, human
  • Heme Oxygenase-1
  • Superoxide Dismutase
  • SIRT1 protein, human
  • SIRT3 protein, human
  • SIRT4 protein, human
  • Sirtuin 1
  • Sirtuin 3
  • Sirtuins