Abstract
RhoA and Rac play key and opposite roles during neuronal polarization. We now show that Lfc, a guanosine nucleotide exchange factor (GEF), localizes to the Golgi apparatus and growth cones of developing neurons and negatively regulates neurite sprouting and axon formation through a Rho signaling pathway. Tctex-1, a dynein light chain implicated in axon outgrowth by modulating actin dynamics and Rac activity, colocalizes and physically interacts with Lfc, thus inhibiting its GEF activity, decreasing Rho-GTP levels, and functionally antagonizing Lfc during neurite formation.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Axons / physiology*
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CHO Cells
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Cell Enlargement
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Cells, Cultured
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Cricetinae
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Cricetulus
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Dyneins / metabolism*
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Golgi Apparatus / metabolism
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Growth Cones / physiology
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Guanine Nucleotide Exchange Factors / genetics
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Guanine Nucleotide Exchange Factors / metabolism*
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Hippocampus / physiology
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Mice
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Neurites / physiology
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Rats
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Signal Transduction
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rhoA GTP-Binding Protein / metabolism
Substances
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Dynlt1 protein, rat
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Guanine Nucleotide Exchange Factors
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Lfc protein, rat
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Dyneins
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rhoA GTP-Binding Protein