Abstract
A FRET assembly reports antibiotic affinities to two different RNA targets. A binder was labeled with a fluorophore that acts both as an acceptor for the emissive nucleoside on the bacterial A-site and a donor fluorophore for the terminally-labeled human A-site. Unlabeled drugs were used to dissociate the labeled antibiotic.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Bacteria / genetics
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Eukaryota
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Fluorescence Resonance Energy Transfer
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Humans
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Nucleic Acid Conformation
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RNA, Bacterial / chemistry
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RNA, Bacterial / metabolism
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RNA, Ribosomal / chemistry
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RNA, Ribosomal / metabolism*
Substances
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Anti-Bacterial Agents
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RNA, Bacterial
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RNA, Ribosomal