Topoisomerase I amplification in melanoma is associated with more advanced tumours and poor prognosis

Pigment Cell Melanoma Res. 2010 Aug;23(4):542-53. doi: 10.1111/j.1755-148X.2010.00720.x. Epub 2010 Apr 30.


In this study, we used array-comparative genomic hybridization (aCGH) and fluorescent in situ hybridization (FISH) to examine genetic aberrations in melanoma cell lines and tissues. Array-comparative genomic hybridization revealed that the most frequent genetic changes found in melanoma cell lines were amplifications on chromosomes 7p and 20q, along with disruptions on Chr 9, 10, 11, 12, 22 and Y. Validation of the results using FISH on tissue microarrays (TMAs) identified TOP1 as being amplified in melanoma tissues. TOP1 amplification was detected in a high percentage (33%) of tumours and was associated with thicker, aggressive tumours. These results show that TOP1 amplification is associated with advanced tumours and poor prognosis in melanoma. These observations open the possibility that TOP1-targeted therapeutics may be of benefit in a particular subgroup of advanced stage melanoma patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Comparative Genomic Hybridization
  • DNA Topoisomerases, Type I / genetics*
  • DNA Topoisomerases, Type I / metabolism
  • Gene Amplification*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Male
  • Melanoma / diagnosis*
  • Melanoma / enzymology*
  • Melanoma / pathology
  • Prognosis


  • DNA Topoisomerases, Type I