Impaired water reabsorption in mice deficient in the type VI adenylyl cyclase (AC6)

FEBS Lett. 2010 Jul 2;584(13):2883-90. doi: 10.1016/j.febslet.2010.05.004. Epub 2010 May 11.


Adenylyl cyclase (AC) type VI (AC6) is a calcium-inhibitable enzyme which produces cAMP upon stimulation. Herein, we characterized the specific role of AC6 in the kidneys using two AC6-knockout mouse lines. Immunohistochemical staining revealed that AC6 exists in the tubular parts of the nephron and collecting duct. Activities of AC evoked by forskolin or a selective agonist of the V2 vasopressin receptor were lower in the kidneys of AC6-null mice compared to those of wildtype mice. Results of a metabolic cage assay and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) showed for the first time that AC6 plays a critical role in regulating water homeostasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylyl Cyclases / genetics
  • Adenylyl Cyclases / metabolism*
  • Animals
  • Cyclic AMP / metabolism
  • Immunohistochemistry
  • Kidney Tubules, Collecting / metabolism
  • Kidney Tubules, Collecting / physiology
  • Magnetic Resonance Imaging
  • Mice
  • Mice, Knockout
  • Reverse Transcriptase Polymerase Chain Reaction
  • Water / metabolism*


  • Water
  • Cyclic AMP
  • Adenylyl Cyclases
  • adenylyl cyclase 6