Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice

EMBO Rep. 2010 Jun;11(6):445-51. doi: 10.1038/embor.2010.64. Epub 2010 May 14.


Heterogeneous nuclear ribonucleoprotein-M (hnRNP-M) is an abundant nuclear protein that binds to pre-mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP-M and the human spliceosome proteins cell division cycle 5-like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the heat-shock stress response. A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. hnRNP-M affects both 5' and 3' alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Cell Cycle Proteins / chemistry
  • Cell Cycle Proteins / metabolism*
  • Green Fluorescent Proteins
  • HeLa Cells
  • Heat-Shock Response
  • Heterogeneous-Nuclear Ribonucleoprotein Group M / chemistry
  • Heterogeneous-Nuclear Ribonucleoprotein Group M / metabolism*
  • Humans
  • Intracellular Signaling Peptides and Proteins / chemistry
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / metabolism*
  • Protein Binding
  • Protein Interaction Mapping
  • Protein Structure, Tertiary
  • RNA Splice Sites*
  • RNA-Binding Proteins / chemistry
  • RNA-Binding Proteins / metabolism*
  • Recombinant Fusion Proteins


  • CDC5L protein, human
  • Cell Cycle Proteins
  • Heterogeneous-Nuclear Ribonucleoprotein Group M
  • Intracellular Signaling Peptides and Proteins
  • Nuclear Proteins
  • PLRG1 protein, human
  • RNA Splice Sites
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins