Abstract
Heterogeneous nuclear ribonucleoprotein-M (hnRNP-M) is an abundant nuclear protein that binds to pre-mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP-M and the human spliceosome proteins cell division cycle 5-like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the heat-shock stress response. A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. hnRNP-M affects both 5' and 3' alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Binding Sites
-
Cell Cycle Proteins / chemistry
-
Cell Cycle Proteins / metabolism*
-
Green Fluorescent Proteins
-
HeLa Cells
-
Heat-Shock Response
-
Heterogeneous-Nuclear Ribonucleoprotein Group M / chemistry
-
Heterogeneous-Nuclear Ribonucleoprotein Group M / metabolism*
-
Humans
-
Intracellular Signaling Peptides and Proteins / chemistry
-
Intracellular Signaling Peptides and Proteins / metabolism*
-
Nuclear Proteins / chemistry
-
Nuclear Proteins / metabolism*
-
Protein Binding
-
Protein Interaction Mapping
-
Protein Structure, Tertiary
-
RNA Splice Sites*
-
RNA-Binding Proteins / chemistry
-
RNA-Binding Proteins / metabolism*
-
Recombinant Fusion Proteins
Substances
-
CDC5L protein, human
-
Cell Cycle Proteins
-
Heterogeneous-Nuclear Ribonucleoprotein Group M
-
Intracellular Signaling Peptides and Proteins
-
Nuclear Proteins
-
PLRG1 protein, human
-
RNA Splice Sites
-
RNA-Binding Proteins
-
Recombinant Fusion Proteins
-
enhanced green fluorescent protein
-
Green Fluorescent Proteins