Structural studies on a novel fucogalactan sulfate extracted from the brown seaweed Laminaria japonica

Int J Biol Macromol. 2010 Aug 1;47(2):126-31. doi: 10.1016/j.ijbiomac.2010.05.010. Epub 2010 May 13.


A low molecular weight fucogalactan, obtained from the brown seaweed Laminaria japonica, was separated into three fractions (LF1, LF2 and LF3) by DEAE-Sepharose FF column chromatography. All three fractions contained predominantly fucose, sulfate group and galactose. The results showed that the main fraction LF2 consisted of l-fucose, d-galactose and sulfate at a molar ratio 6:1:9. Structural study on the LF2 was carried out by NMR spectroscopy. The backbone of LF2 was primarily (1-->3)-linked alpha-l-fucopyranose residues (75%) and a few (1-->4)-alpha-l-fucopyranose linkages (25%). The branch points were at C-4 of 3-linked alpha-l-fucopyranose residues by beta-d-galactopyranose unites (35%, molar ratio) or at C-2 of 3-linked alpha-l-fucopyranose residues by non-reducing terminal fucose unites (65%, molar ratio). Sulfate groups occupied at position C-4 or C-2, sometimes C-2, 4 to fucose residues, and C-3 and/or C-4 to galactose residues. The structure of LF2 was supposed as following: .

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Chemical Fractionation
  • Galactans / chemistry*
  • Galactans / isolation & purification*
  • Laminaria / chemistry*
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data


  • Galactans