Inflammatory responses improve with milk ribonuclease-enriched lactoferrin supplementation in postmenopausal women

Inflamm Res. 2010 Nov;59(11):971-8. doi: 10.1007/s00011-010-0211-7. Epub 2010 May 15.


Objective and design: A 6-month, randomized clinical study was conducted to evaluate the effect of a ribonuclease-enriched lactoferrin (R-ELF) supplement on the circulating cytokine levels and bone health of postmenopausal women.

Subjects: Thirty-eight healthy postmenopausal women, aged 45-60 years, were randomized into placebo and R-ELF groups.

Treatment: The R-ELF group was supplemented with R-ELF (2 × 125 mg/day) and calcium (100% RDA), while the placebo group received only the calcium supplement.

Methods: Serum levels of receptor activator for NF-κB ligand (RANKL), C-reactive protein (CRP) and various pro- and anti-inflammatory cytokines were determined by ELISA.

Results: Pro-inflammatory cytokines IL-6 and TNF-α decreased significantly (-44 and -10%, respectively) while anti-inflammatory IL-10 increased (140%) due to R-ELF supplementation at the end of study. RANKL and CRP were modestly reduced (-50%) relative to their placebo levels, although RANKL elevated initially.

Conclusions: R-ELF supplementation showed beneficial effects towards improvement of inflammatory status in postmenopausal women.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cytokines / blood
  • Cytokines / immunology
  • Dietary Supplements*
  • Female
  • Humans
  • Inflammation / blood
  • Inflammation / immunology*
  • Lactoferrin* / administration & dosage
  • Lactoferrin* / chemistry
  • Middle Aged
  • Milk / enzymology*
  • Placebos
  • Postmenopause*
  • Ribonucleases / administration & dosage*


  • Cytokines
  • Placebos
  • Ribonucleases
  • Lactoferrin