Analysis of early human neural crest development

Dev Biol. 2010 Aug 15;344(2):578-92. doi: 10.1016/j.ydbio.2010.05.012. Epub 2010 May 15.

Abstract

The outstanding migration and differentiation capacities of neural crest cells (NCCs) have fascinated scientists since Wilhelm His described this cell population in 1868. Today, after intense research using vertebrate model organisms, we have gained considerable knowledge regarding the origin, migration and differentiation of NCCs. However, our understanding of NCC development in human embryos remains largely uncharacterized, despite the role the neural crest plays in several human pathologies. Here, we report for the first time the expression of a battery of molecular markers before, during, or following NCC migration in human embryos from Carnegie Stages (CS) 12 to 18. Our work demonstrates the expression of Sox9, Sox10 and Pax3 transcription factors in premigratory NCCs, while actively migrating NCCs display the additional transcription factors Pax7 and AP-2alpha. Importantly, while HNK-1 labels few migrating NCCs, p75(NTR) labels a large proportion of this population. However, the broad expression of p75(NTR) - and other markers - beyond the neural crest stresses the need for the identification of additional markers to improve our capacity to investigate human NCC development, and to enable the generation of better diagnostic and therapeutic tools.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation / genetics
  • Humans
  • Neural Crest / cytology*
  • Neural Crest / metabolism*
  • Neurons / cytology*
  • Neurons / metabolism
  • PAX7 Transcription Factor / genetics
  • PAX7 Transcription Factor / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*

Substances

  • PAX7 Transcription Factor
  • Transcription Factors