Ameliorating effect of chicory (Cichorium intybus L.)-supplemented diet against nitrosamine precursors-induced liver injury and oxidative stress in male rats

Food Chem Toxicol. 2010 Aug-Sep;48(8-9):2163-9. doi: 10.1016/j.fct.2010.05.023. Epub 2010 May 15.


The current study was carried out to elucidate the modulating effect of chicory (Cichorium intybus L.)-supplemented diet against nitrosamnine-induced oxidative stress and hepatotoxicity in male rats. Rats were divided into four groups and treated for 8 weeks as follow: group 1 served as control; group 2 fed on chicory-supplemented diet (10% w/w); group 3 received simultaneously nitrosamine precursors [sodium nitrite (0.05% in drinking water) plus chlorpromazine (1.7 mg/kg body weight)] and group 4 received nitrosamine precursors and fed on chicory-supplemented diet. The obtained results revealed that rats received nitrosamine precursors showed a significant increase in liver TBARS and total lipids, total cholesterol, bilirubin, and enzymes activity (AST, ALT, ALP and gamma-GT) in both serum and liver. While a significant decrease in the levels of GSH, GSH-Rx, SOD, catalase, total protein and albumin was recorded. On the other hand, chicory-supplemented diet succeeded to modulate these observed abnormalities resulting from nitrosamine compounds as indicated by the reduction of TBARS and the pronounced improvement of the investigated biochemical and antioxidant parameters. So, it could be concluded that chicory has a promising role and it worth to be considered as a natural substance for ameliorating the oxidative stress and hepatic injury induced by nitrosamine compounds.

MeSH terms

  • Animals
  • Blood Chemical Analysis
  • Chemical and Drug Induced Liver Injury / prevention & control*
  • Chicory / chemistry*
  • Chlorpromazine / pharmacology
  • Diet
  • Dietary Supplements*
  • Dopamine Antagonists / pharmacology
  • Dose-Response Relationship, Drug
  • Indicators and Reagents
  • Liver / drug effects
  • Liver / metabolism
  • Liver Function Tests
  • Male
  • Nitrosamines / antagonists & inhibitors*
  • Nitrosamines / toxicity*
  • Oxidative Stress / drug effects*
  • Rats
  • Rats, Sprague-Dawley
  • Thiobarbituric Acid Reactive Substances / metabolism


  • Dopamine Antagonists
  • Indicators and Reagents
  • Nitrosamines
  • Thiobarbituric Acid Reactive Substances
  • Chlorpromazine