Abstract
Inflammation is part of the host defense mechanism against harmful matters and injury; however, aberrant inflammation is associated to the development of chronic disease such as cancer. Raspberry ketone is a natural phenolic compound. It is used in perfumery, in cosmetics, and as a food additive to impart a fruity odor. In this study, we evaluated whether rheosmin, a phenolic compound isolated from pine needles regulates the expression of iNOS and COX-2 protein in LPS-stimulated RAW264.7 cells. Rheosmin dose-dependently inhibited NO and PGE(2) production and also blocked LPS-induced iNOS and COX-2 expression. Rheosmin potently inhibited the translocation of NF-kappaB p65 into the nucleus by IkappaB degradation following IkappaB-alpha phosphorylation. This result shows that rheosmin inhibits NF-kappaB activation. In conclusion, our results suggest that rheosmin inhibits LPS-induced iNOS and COX-2 expression in RAW264.7 cells by blocking NF-kappaB activation pathway.
Copyright (c) 2010 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / analysis
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Blotting, Western
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Butanones / analysis
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Butanones / pharmacology*
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Cyclooxygenase 2 / biosynthesis*
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Cyclooxygenase 2 Inhibitors / pharmacology*
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Dinoprostone / biosynthesis
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Electrophoresis, Polyacrylamide Gel
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I-kappa B Proteins / metabolism
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Immunohistochemistry
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Lipopolysaccharides / antagonists & inhibitors*
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Macrophages / metabolism
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Mice
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Molecular Weight
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NF-kappa B / drug effects*
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Nitric Oxide / biosynthesis
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Nitric Oxide Synthase Type II / biosynthesis*
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Phenols / analysis
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Phenols / pharmacology*
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Phosphorylation
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Pinus / chemistry*
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Signal Transduction / drug effects
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Transcription Factor RelA / biosynthesis
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Butanones
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Cyclooxygenase 2 Inhibitors
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I-kappa B Proteins
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Lipopolysaccharides
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NF-kappa B
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Phenols
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Transcription Factor RelA
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rheosmin
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Nitric Oxide
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Nitric Oxide Synthase Type II
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Cyclooxygenase 2
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Dinoprostone