Sugar transporters of the major facilitator superfamily in aphids; from gene prediction to functional characterization

Insect Mol Biol. 2010 Mar;19 Suppl 2:97-112. doi: 10.1111/j.1365-2583.2009.00918.x.

Abstract

Analysis of the pea aphid (Acyrthosiphon pisum) genome using signatures specific to the Major Facilitator Superfamily (Pfam Clan CL0015) and the Sugar_tr family (Pfam Family PF00083) has identified 54 genes encoding potential sugar transporters, of which 38 have corresponding ESTs. Twenty-nine genes contain the InterPro IPR003663 hexose transporter signature. The protein encoded by Ap_ST3, the most abundantly expressed sugar transporter gene, was functionally characterized by expression as a recombinant protein. Ap_ST3 acts as a low-affinity uniporter for fructose and glucose that does not depend on Na(+) or H(+) for activity. Ap_ST3 was expressed at elevated levels in distal gut tissue, consistent with a role in gut sugar transport. The A. pisum genome shows evidence of duplications of sugar transporter genes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aphids / genetics*
  • Aphids / metabolism*
  • Base Sequence
  • DNA Primers / genetics
  • Evolution, Molecular
  • Expressed Sequence Tags
  • Fructose / metabolism
  • Gene Duplication
  • Genes, Insect*
  • Genome, Insect
  • Glucose / metabolism
  • Insect Proteins / genetics*
  • Insect Proteins / metabolism*
  • Kinetics
  • Monosaccharide Transport Proteins / genetics*
  • Monosaccharide Transport Proteins / metabolism*
  • Multigene Family*
  • Peas / parasitology
  • Phylogeny
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Species Specificity

Substances

  • DNA Primers
  • Insect Proteins
  • Monosaccharide Transport Proteins
  • Recombinant Proteins
  • Fructose
  • Glucose