Early treatment of transient focal cerebral ischemia with bovine PEGylated carboxy hemoglobin transfusion

Artif Cells Blood Substit Immobil Biotechnol. 2010 Oct;38(5):223-9. doi: 10.3109/10731199.2010.488635.


The effect of transfusion of PEGylated hemoglobin (PEG-Hb) was evaluated in anesthetized rats subjected to 2 hours of focal cerebral ischemia and 1 day of reperfusion. PEG-Hb was stored in the carboxy state (PEG-COHb) to reduce autooxidation and increase the shelf life. Transfusion of 10 ml/kg of PEG-COHb at 20 minutes of ischemia did not alter arterial blood pressure or increase red cell flux in the ischemic core. Plasma hemoglobin increased to only 0.6 g/dL, yet infarct volume was markedly decreased and neurological deficits were improved. We conclude that early topload transfusion of PEG-COHb protects the brain from ischemic stroke.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Blood Gas Analysis
  • Blood Pressure
  • Blood Transfusion / methods*
  • Body Temperature
  • Carboxyhemoglobin / chemistry*
  • Carboxyhemoglobin / therapeutic use*
  • Cattle
  • Electrolytes / blood
  • Ischemic Attack, Transient / blood
  • Ischemic Attack, Transient / physiopathology
  • Ischemic Attack, Transient / therapy*
  • Male
  • Polyethylene Glycols / chemistry*
  • Rats
  • Rats, Wistar
  • Time Factors


  • Electrolytes
  • Polyethylene Glycols
  • Carboxyhemoglobin