Anti-inflammatory effect of enzymatic hydrolysate of corn gluten in an experimental model of colitis

J Pharm Pharmacol. 2010 Mar;62(3):389-92. doi: 10.1211/jpp.62.03.0015.


Objectives: Intestinal bacteria are thought to be involved in the initiation and perpetuation of inflammatory bowel diseases. Prebiotics (non-digestable dietary carbohydrate) have beneficial properties that alter the intestinal flora and contain glutamine-rich protein. Glutamine significantly decreases indices of inflammation. In this study, an enzymatic hydrolysate of corn gluten (EHCG) was administered by gavage to Sprague-Dawley rats fed an elemental diet to determine whether EHCG can ameliorate experi- mental colitis.

Methods: Colitis was induced by intrarectal administration of 2,4,6-trinitrobenzene sulfonic acid after 10 days' daily oral administration of EHCG at 100 and 300 mg/kg. Macroscopic damage was assessed using a scoring system. The mucosa homogenate was sonicated and myeloperoxidase activity and histamine levels measured.

Key findings: Treatment with EHCG significantly decreased the severity of injury and reduced myeloperoxidase activity and histamine levels in the distal colon mucosa.

Conclusions: EHCG may have therapeutic benefit as a supplement in enteral nutrition for patients with inflammatory bowel diseases.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / administration & dosage
  • Anti-Inflammatory Agents, Non-Steroidal / therapeutic use*
  • Colitis / chemically induced
  • Colitis / pathology
  • Colitis / prevention & control*
  • Colon, Descending / drug effects
  • Colon, Descending / metabolism
  • Colon, Descending / pathology
  • Dietary Supplements
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Food, Formulated / adverse effects
  • Glutens / metabolism*
  • Histamine / metabolism
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / metabolism
  • Intestinal Mucosa / pathology
  • Male
  • Peroxidase / metabolism
  • Protein Hydrolysates / administration & dosage
  • Protein Hydrolysates / therapeutic use*
  • Rats
  • Rats, Sprague-Dawley
  • Seeds / chemistry*
  • Severity of Illness Index
  • Trinitrobenzenesulfonic Acid / toxicity
  • Zea mays / chemistry*


  • Anti-Inflammatory Agents, Non-Steroidal
  • Protein Hydrolysates
  • Glutens
  • Histamine
  • Trinitrobenzenesulfonic Acid
  • Peroxidase