Abstract
Hepatitis B virus DNA was extracted from serum of a chronic carrier and polymerase chain reaction was performed on S gene. Direct sequencing showed a variant HBsAg with additional 4-amino acid insertion, and clone sequencing confirmed the mixture of variant HBsAg and wildtype HBsAg. Of 16 clones with 12-nucleotide insertion, 15 clones had identical AGAACAACACAA insertion between nucleotide 494 and nucleotide 495, and one clone had GGAACAACTCAA insertion in the same position plus 3-nucleotide deletion from nucleotide 491 to nucleotide 493. S114T, C121Y, T126S/A, Q129K, G130R, T131N, M133T, G145R, N146D substitution and premature stop codon were also found in those clones. However, the origin of HBV with 4-amino acid insertion in HBsAg was unclear.
MeSH terms
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Aged
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Amino Acid Sequence
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Base Sequence
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Carrier State / virology*
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Hepatitis B Surface Antigens / chemistry
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Hepatitis B Surface Antigens / genetics*
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Hepatitis B virus / chemistry
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Hepatitis B virus / classification
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Hepatitis B virus / genetics*
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Hepatitis B virus / isolation & purification
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Hepatitis B, Chronic / virology*
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Humans
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Male
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Molecular Sequence Data
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Mutagenesis, Insertional*
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Phylogeny
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Sequence Alignment
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Sequence Deletion
Substances
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Hepatitis B Surface Antigens
Associated data
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GENBANK/GU145104
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GENBANK/GU358454
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GENBANK/GU358455
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GENBANK/GU358456
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GENBANK/GU358457
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GENBANK/GU358458
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GENBANK/GU358459
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GENBANK/GU358460
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GENBANK/GU358461
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GENBANK/GU358462
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GENBANK/GU358463
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GENBANK/GU358464
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GENBANK/GU358465
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GENBANK/GU358466
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GENBANK/GU358467
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GENBANK/GU358468
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GENBANK/GU358469
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GENBANK/GU358470
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GENBANK/GU358471
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GENBANK/GU358472
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GENBANK/GU358473