Dectin-2 recognition of alpha-mannans and induction of Th17 cell differentiation is essential for host defense against Candida albicans

Immunity. 2010 May 28;32(5):681-91. doi: 10.1016/j.immuni.2010.05.001. Epub 2010 May 20.


Dectin-2 (gene symbol Clec4n) is a C-type lectin expressed by dendritic cells (DCs) and macrophages. However, its functional roles and signaling mechanisms remain to be elucidated. Here, we generated Clec4n(-/-) mice and showed that this molecule is important for host defense against Candida albicans (C. albicans). Clec4n(-/-) DCs had virtually no fungal alpha-mannan-induced cytokine production. Dectin-2 signaling induced cytokines through an FcRgamma chain and Syk-CARD9-NF-kappaB-dependent signaling pathway without involvement of MAP kinases. The yeast form of C. albicans induced interleukin-1beta (IL-1beta) and IL-23 secretion in a Dectin-2-dependent manner. In contrast, cytokine production induced by the hyphal form was only partially dependent on this lectin. Both yeast and hyphae induced Th17 cell differentiation, in which Dectin-2, but not Dectin-1, was mainly involved. Because IL-17A-deficient mice were highly susceptible to systemic candida infection, this study suggests that Dectin-2 is important in host defense against C. albicans by inducing Th17 cell differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Candida albicans / immunology*
  • Candidiasis / immunology*
  • Cell Differentiation*
  • Cells, Cultured
  • Immunoassay
  • Interleukin-17 / metabolism*
  • Interleukin-1beta / immunology
  • Interleukin-23 / immunology
  • Lectins, C-Type / genetics
  • Lectins, C-Type / immunology*
  • Male
  • Mannans / immunology*
  • Mice
  • Mice, Knockout
  • Signal Transduction
  • T-Lymphocytes, Helper-Inducer* / cytology
  • T-Lymphocytes, Helper-Inducer* / immunology


  • Interleukin-17
  • Interleukin-1beta
  • Interleukin-23
  • Lectins, C-Type
  • Mannans
  • dectin-2, mouse