Halomarina oriensis gen. nov., sp. nov., a halophilic archaeon isolated from a seawater aquarium

Int J Syst Evol Microbiol. 2011 Apr;61(Pt 4):942-946. doi: 10.1099/ijs.0.020677-0. Epub 2010 May 21.

Abstract

A novel halophilic archaeon, strain KeC-11(T), was isolated from a seawater aquarium at the Ocean Research Institute, University of Tokyo, Japan. The strain was aerobic, Gram-negative and chemo-organotrophic, growing optimally at 37 °C, at pH 7.0-8.0 and in 2.7 M (16 %) NaCl. The strain required at least 10 mM magnesium ions for growth. Cells of strain KeC-11(T) were non-motile and generally irregular coccoids or discoids. The DNA G+C content of the isolate was 67.7 mol%. Phylogenetic tree reconstructions indicated that it was distantly related to the other recognized members of the family Halobacteriaceae, with the closest relative being Natronomonas pharaonis Gabara(T) (91 % sequence similarity). The strain contained C(20)C(20) and C(20)C(25) diether derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, a glycolipid chromatographically identical to the glycosyl-mannosyl-glucosyl diether (TGD-2) and at least one unidentified glycolipid. Phenotypic characterization and phylogenetic data support the placement of isolate KeC-11(T) in a novel species in a new genus within the family Halobacteriaceae, for which the name Halomarina oriensis gen. nov., sp. nov. is proposed; the type strain is KeC-11(T) ( = JCM 16495(T) = KCTC 4074(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aerobiosis
  • Base Composition
  • Cluster Analysis
  • DNA, Archaeal / chemistry
  • DNA, Archaeal / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Glycolipids / analysis
  • Halobacteriaceae / classification*
  • Halobacteriaceae / genetics
  • Halobacteriaceae / isolation & purification*
  • Halobacteriaceae / physiology
  • Hydrogen-Ion Concentration
  • Japan
  • Magnesium / metabolism
  • Molecular Sequence Data
  • Organic Chemicals / metabolism
  • Phospholipids / analysis
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Sodium Chloride / metabolism
  • Temperature

Substances

  • DNA, Archaeal
  • DNA, Ribosomal
  • Glycolipids
  • Organic Chemicals
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Sodium Chloride
  • Magnesium

Associated data

  • GENBANK/AB519798